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Professor
Director of Core Facilities
President of The American Association
of Anatomists
(214) 828-8487 Room: 430
ksvoboda@bcd.tamhsc.edu
Cell-Matrix Interaction in Developing
Cartilage and Corneal Epithelia. |
Dr Kathy Svoboda [view
profile]
has been interested in the role of the cytoskeleton in cell shape changes
throughout her career. She studied neuroepithelial cell shape changes
during optic vesicle formation for her Ph.D. thesis topic, and then started
studying the corneal epithelial response to extracellular matrix as a
post doctoral project.
She is presently working on several projects that
have the long-term objective of understanding cell-matrix communication
in whole tissue development models. Her group has established that
just as cultured cells form focal adhesions in response to extracellular
matrix proteins, whole tissues also have similar structures termed cell-matrix
attachment complexes (CMAX). Both the focal adhesion and CMAX contain
cell adhesion molecules, actin associated proteins and signaling molecules.
This group has shown that these proteins and activated signaling pathways
are necessary for reorganizing actin in the embryonic corneal epithelial
model. They have also shown that cell-matrix interactions in whole
cartilage are necessary for survival and differentiation.
Understanding
these relationships will help elucidate the events and interactions
that are involved in tissue specific differentiation and matrix synthesis.
Her group has developed experimental approaches to examine the spatial
relationships between specific cellular components in whole tissues.
These cellular models have been used to determine the three dimensional
relationships between organelles, cytoskeletal proteins and specific
mRNA.
In 1998 Dr. Svoboda joined the faculty at Baylor College of Dentistry.
Although she is still pursuing the long-term goals of her research, in
the last seven years she has become involved in many other projects.
The new projects (signal transduction pathways controlling palate development,
condylar cartilage differentiation and gingival tissue response to nicotine)
are related to craniofacial development or cell-matrix interactions in
oral tissues.
Dr. Svoboda has served on the executive board and been the program secretary
for the American Association of Anatomists and will be president from 2005-7
(www.anatomy.org). Dr. Svoboda has
also serves on the editorial board of Developmental Dynamics and is an
associate editor for Anatomical Record.
The image to the right
was the cover page in IOVS journal (Volume 43, Issue 10).
This is a single confocal optical section of the actin cortical mat from embryonic
chicken corneal epithelia that reorganized after type I collagen stimulation
(Reenstra et al., Fig. 2A, 2p. 3185).
The epithelia were isolated as a sheet of cells
without the basal lamina, and then incubated in the presence of control
oligonucleotidess (Rho sense) and collagen.
The images were pseudo-colored using a scale
based on intensity of F-actin (phalloidin) staining.
The least intense area
were designated blue and the most intense areas were designated red.
Harvard Medical School, Boston, Massachusetts
Postdoctoral followship (1982-1985) Department of Anatomy & Cell
Biology
University of Nebraska Medical Center, Omaha, Nebraska
Ph.D. (1982) The Department of Anatomy
University of Nebraska Medical Center, Omaha, Nebraska
M.S. (1979) The Department of Human Genetics
University of Nebraska, Omaha, Nebraska
B.S. (1974)
| 2001-present |
Professor, Department of Biomedical Sciences,
Baylor College of Dentistry; Texas A&M University System Health
Science Center |
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| 2001-present |
Adjunct Professor, Department of Ophthalmology,
Southwestern Medical Center, Dallas, TX |
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| 1999-present |
Director of the Cell and Molecular Biology Core Facilities
Baylor College of Dentistry; Texas A&M University System Health
Science Center |
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| 1998-2001 |
Associate Professor, Department of Biomedical Sciences,
Baylor College of Dentistry; Texas A&M University System Health
Science Center |
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| 1996-1998 |
Associate Professor, Department of Ophthalmology
Boston University School of Medicine, Boston, Massachusetts |
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| 1994-1998 |
Associate Professor of Anatomy and Neurobiology
Boston University School of Medicine, Boston, Massachusetts |
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| 1992-1998 |
Co-Director of the Confocal Facility,
Boston University School of Medicine, Boston, Massachusetts |
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| 1987-1994 |
Assistant Professor of Anatomy and Neurobiology
Boston University School of Medicine, Boston, Massachusetts |
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| 1985-1986 |
Instructor in Anatomy and Cellular Biology
Harvard Medical School, Boston, Massachusetts |
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Prenatal Craniofacial Development (director), Cell and Molecular Biology
of Oral and Craniofacial Tissues (director), Cell and Molecular Biology
for D1 students (director) and General Histology.
- Biology of the Embryonic
Corneal Epithelium. NIH RO1, 1991-2003.The
effects of nicotine on plaate development. Texas A&M HSC
Tobacco Endowment Fund Award, 2000-2002.
- The Effects of Nicotine on Palate Development. Texas
A&M Tobacco Endowment Award, 2000-2002.
- Individual Predoctoral Dental Scientist Award. For
David Erik Kern, 2000-2006. (mentor)
- Acquisition
of Laser Capture Microdissection and Quantitative PCR Systems.
 (File
Size: 55.1 KB) NSF Award, 2003-2006.
- Plasmid Delivery and Expression in
Embryonic Eye Tissues. (File
Size: 39.9KB) NIH RO3 Award, 2003-2006.
- Research Infrastructure Improvement
Planning Award. NIH R24,
Co PI, 2003-2004
- BCD Research Infrastructure Enhancement
Program Award. [website]
NIH U24, Co PI, 2004-2006
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Cornea Epithelia Project:
- Chu, C.L., W.R. Reenstra, and K.K.H.
Svoboda, (2000) Extracellular
signal-regulated kinase and PI3 kinase are necessary
for collagen binding and actin reorganization in avian
corneal epithelia. (File
Size: 5.25MB) Invest. Ophthal. and Vis. Sci., 41: 3374-3382.
- Svoboda,
K.K.H. and W.R. Reenstra, (2002) Approaches
To Studying Cellular Signaling: A Primer For Morphologists. (File
Size: 1.98MB) Anatomical Record, The New Anatomist, 269:123-13
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Watanabe, M., Hitomi,
M., van der Wee, K., Rothenberg, F., Fisher, S.A., Zucker,
R., Svoboda, K.K.H., Goldsmith, E.C. and Nieman, A-L. (2002)
The pros and cons of apoptosis assays for use in the study
of tissues and organs. Microscopy and Microanalysis 8:5, 375-391. Cover
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Reenstra, W.R., D.L. Orlow, and
K.K.H. Svoboda, (2002) ECM
Stimulated Signaling And Actin Reorganization
In Embryonic Corneal Epithelia Are Rho Dependent. (File
Size: 793KB) Invest. Ophthal. and Vis. Sci., 48: 3185. Cover.
- Svoboda, K.K.H., Moessner, P., Field, T., Acevedo, J. 2004
The Rho-associated protein kinase inhibitor Y27632 increases
apoptosis and blocks actin cortical mat reformation in embryonic
corneal epithelia. Developmental Dynamics 229: 579-590. (link
to pdf file #1) (File
Size: 1.29 MB)
Palate
Project:
- Kang
P. and Svoboda K. K. H. (2002) PI3 Kinase Activity
is Essential for Epithelial-Mesenchymal Transformation during
Murine Palate Fusion. Developmental Dynamics, (in press).
- Kang
P. and Svoboda K. K. H. (2002) Nicotine Inhibits
Palatal Fusion and Modulates Nicotinic Receptors and the
PI-3 Kinase Pathway in Medial Edge Epithelia (submitted).
- Kang, P. and Svoboda, K.K.H. (2005) Epithelial-Mesenchymal
Transformation during Craniofacial Development. Journal of Dental
Research (accepted, in revision).
- Spears, R. and Svoboda, K.K.H. (2005) Growth Factors and Signaling Proteins in Craniofacial
Development. Seminars in Orthodontics.
(accepted, in press).
Cartilage
Development Project:
- Hirsch,
M.S., L.E. Lunsford, V. Trinkaus-Randall and K.K.H.
Svoboda.
(1997) Chondrocyte survival
and differentiation in situ are integrin mediated. (File
Size: 759KB) Developmental Dynamics 210: 249-263.
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Hirsch,
M.S. and K.K.H.
Svoboda. (1998) Establishment of
a whole organ culture model that recapitulates normal
cartilage development. BioTechniques, (April
cover image) 24 (4) 632-636.
- Svoboda, K.K.H. (1998)Chondrocyte-Matrix
attachment complexes mediate survival differentiation. (File
Size: 467KB) Microsc. Res. Tech. 43:111-122.
- Harrington,
E. K., Lunsford, L. E. and Svoboda,
K.K.H. (2004)
PTH Modulates Chondrocyte Survival, Differentiation, Type X Collagen
Deposition and mRNA Expression in vitro. Anatomical Record 281A:
1286-1295. (File
Size: 265 KB)
Gingival
Fibroblast Project:
- Fang, Y. and Svoboda,
K.K.H. (2005) Nicotine
Inhibits Myofibroblast Differentiation in Human Gingival Fibroblasts.
(in revision).
- Fang, Y. and Svoboda,
K.K.H. (2005) Nicotine
Inhibits Human Gingival Fibroblast Migration via Modulation of
Rac Signaling Pathways. (submitted).
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